The incubation time in liquid paraffin need to be lengthy sufficient to get enough infiltration, although not any longer than necessary in an effort to avoid extended publicity from the tissue to heat. For the same cause, it is suggested which the temperature of your liquid paraffin need to be not more than 2–four C over the paraffin's melting le
As we discussed previously, fatty tissue is essentially challenging to penetrate, so when picking your representative piece of tissue, you have got to keep in mind that some trimming will be required. You should have the specimen all the way down to an excellent sizing. Optimally Now we have one to two-millimeter biopsies for rapid processing , and
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